Rt Pcr Data Analysis. Real time pcr has become a widely used technique for a variety of applications such as gene expression mutational analysis and pathogen detection many biotechnology companies offer real time pcr instruments with data analysis software packages to assist with these applications. Real time pcr requires an instrumentation platform that consists of a thermal cycler a computer optics for fluorescence excitation and emission collection and data acquisition and analysis software these machines available from several manufacturers differ in sample capacity some are 96 well standard format others process fewer samples or require specialized glass capillary tubes.
This is achieved by monitoring the amplification reaction using. Real time pcr has become a widely used technique for a variety of applications such as gene expression mutational analysis and pathogen detection many biotechnology companies offer real time pcr instruments with data analysis software packages to assist with these applications. It is primarily used to measure the amount of a specific rna.
Real time pcr requires an instrumentation platform that consists of a thermal cycler a computer optics for fluorescence excitation and emission collection and data acquisition and analysis software these machines available from several manufacturers differ in sample capacity some are 96 well standard format others process fewer samples or require specialized glass capillary tubes.
Reverse transcription polymerase chain reaction rt pcr is a laboratory technique combining reverse transcription of rna into dna in this context called complementary dna or cdna and amplification of specific dna targets using polymerase chain reaction pcr. From ct analysis of your real time pcr gene expression experiments to allele cluster analysis of applied biosystems taqman snp assay data to cnv copy number determination to hrm curve generation we create software that enables you to easily analyze data from your real time pcr instrument. This is achieved by monitoring the amplification reaction using. Real time pcr requires an instrumentation platform that consists of a thermal cycler a computer optics for fluorescence excitation and emission collection and data acquisition and analysis software these machines available from several manufacturers differ in sample capacity some are 96 well standard format others process fewer samples or require specialized glass capillary tubes.
