Positive Indirect Elisa Test. Therefore one binding can make a strong signal due to more than one interactions. A known antigen an unknown antibody and a known antibody c.
In an elisa four types of false positive reactions can be encountered regardless of the antigens coated on the elisa plate. A positive indirect elisa result requires a. Unknowns that generate a signal that is stronger than the known sample are positive and those that generate weaker signal are negative.
This color is directly proportional to the amount of bound sample antibody.
Two known antibodies and one unknown antigen. An example of an indirect elisa test is the human immunodeficiency virus test used to detect antibodies against hiv. 1 non specific reaction caused by the secondary antibody 2 hydrophobic binding of immunoglobulin components in sample specimens to plastic surfaces 3 ionic interaction between immunoglobulin in sample specimens and antigen and 4 immune recognition of blocking agents by antibodies in serum specimens e g. However the western blot is no longer used and today.
