Indirect Elisa Test Procedure. Then this antigen containing buffer solution added to a microtiter plate a specific plate containing multiple wells. After incubation uncover the micro titer plate.
Elisa is a rapid test used for detecting or quantifying antibody ab against viruses bacteria and other materials or antigen ag. Procedure of indirect elisa coat the micro titer plate wells with antigen. Direct elisa test at first the test antigen the antigen you have chosen for the elisa test mixed with a buffer solution.
After incubation uncover the micro titer plate.
Direct indirect sandwich or competitive. Direct elisa test uses only primary antibodies to detect the antigen while indirect elisa uses both primary and secondary antibodies. Elisa is so named because the test technique involves the use of an enzyme system and immunosorbent. Samples with antibodies are added and washed.
